Assessment of VDR Gene Polymorphism (FokI) in Epithelial Ovarian Cancer Using PCR-RFLP Method

Authors

  • Kanishka Uthansingh KIMS Cancer Hospital, Kalinga Institute of Medical Sciences (KIMS), KIIT University, Patia, Bhubaneswar, 751024, India https://orcid.org/0000-0002-2465-0839
  • Sanghamitra Mishra Institute of Medical Sciences and SUM Hospital, SOA deemed to be University, India
  • Basanta Kumar Pati SUM Ultimate Medicare, SOA deemed to be University, India https://orcid.org/0000-0001-9392-8446

DOI:

https://doi.org/10.47723/jbamm432

Keywords:

VDR, Epithelial ovarian cancer, Genotyping, PCR-RFLP

Abstract

Background: Epithelial ovarian cancer (EOC) has been associated with genetic variations in the vitamin D receptor (VDR) gene among women in different geographical locations. Due to inadequate detection methods, many cases are diagnosed at advanced stages. Vitamin D deficiency and the FokI gene polymorphism in the VDR gene are known to increase cancer risk. The current study aimed to evaluate the correlation between the VDR gene polymorphism (FokI) and epithelial ovarian cancer risk

Subjects and Methods: A prospective case-control study with 160 participants comprised 80 patients with epithelial ovarian cancer and 80 controls without the disease. FokI genotyping was observed using the PCR-RFLP technique, which allows for the precise identification of genetic variants in the VDR gene. Additionally, vitamin D levels were assessed through chemiluminescence immunoassay, providing quantitative data on vitamin D status.

Results: Serum vitamin D levels showed no clear association with ovarian cancer risk across tertiles (p > 0.05). Genetic analysis identified significant genotype distributions in cases versus controls, with the CC genotype associated with reduced risk (OR = 0.5238, 95% CI: 0.042 - 0.5238) and the CT genotype with increased risk (OR = 1.9091, 95% CI: 0.047 - 1.9091) of ovarian cancer.

Conclusions: Findings underscore the multifaceted nature of ovarian cancer etiology, suggesting that CT genotype in FOK1 gene increases the risk of ovarian cancer

Author Biographies

  • Kanishka Uthansingh, KIMS Cancer Hospital, Kalinga Institute of Medical Sciences (KIMS), KIIT University, Patia, Bhubaneswar, 751024, India

    Dr. Kanishka Uthansingh is a Senior Associate Researcher working in Institute of Medical Sciences and SUM Hospital under Siksha O Anusandhan deemed to be University, Bhubaneswar. He has seamlessly bridged the gap between experimental, clinical, and computational biology during the entire research on different gastrointestinal conditions. He has spearheaded diverse interdisciplinary projects throughout his scholarly career as a molecular biologist and biomedical researcher. His Ph.D. equipped him with expertise molecular experimental work, bioinformatics data analysis, clinical data analysis through statistical and mathematical techniques, allowing him to develop non-invasive diagnostic marker such as COX-2 and TNF alpha, and CYP2C9 gene mutation as risk factors for Gastric cancer. 

     

    His collaborative efforts have resulted in several noteworthy discoveries, including:

    1. Non-invasive diagnostic marker discovery a panel of COX-2 marker (Cox-2 1195GA, -765GC, and -1290AG) which was presenter award in an international event (APDW-2019), where the work was praised by the international faculties from different countries.
    1. Therapeutic pathway discovery:  Dr. Uthansingh as a scholar was involved extensively working on the functional interaction (positive or negative) between Wnt/β-catenin and Hedgehog (Hh) signaling pathways in HCC using different models of HCC and also in the bio specimens from HCC patients.
    2. Drug discovery and work on computational and statistical implications: He has explored a novel discovery i.e., fluoroquinolones and tetracycline through interdisciplinary clinical and computational approach revealing the most efficient drug for the treatment of Helicobacter pylori infection..
    3. Community work: Furthermore, He was associated with awareness and vaccination for the infection of Hepatitis B among under privileged people in Odisha associating with Gastro and liver care foundation of India.

    The above significant contributions have been published in different International peered reviewed Journals.

  • Sanghamitra Mishra, Institute of Medical Sciences and SUM Hospital, SOA deemed to be University, India

    Prof. S Mishra is working as Dean, faculty of Medical Sciences, IMS and SUM Hospital. She worked in various capacities like, additional dean, post graduate studies, professor & hod, department of critical care medicine, professor, department of anaesthesiology, pain, Palliative & Critical care Medicine, IMS & SUM Hospital, SOA University,BBSR

  • Basanta Kumar Pati, SUM Ultimate Medicare, SOA deemed to be University, India

    Prof. Basanta Kumar Pati is currently, working as Director, SUM Ultimate Medicare, Professor at IMS & SUM Hospital. Prof. Pati is an Gynecologist and Obstetricians in Aiginia in Bhubaneswar. He is a renowned and highly trusted personality in the medical fraternity. Dr. Basanta Kumar Pati owns rich experience in Obstetrics & Gynaecology. Dr. Basanta Kumar Pati offers some advanced medical treatments for Dysmenorrhea, Female Sexual Dysfunction, Ovarian Cancer, Fibroids, Infertility etc.
    Looking at his ever-increasing popularity, one thing is for sure that he is one of the best trusted doctors in Bhubaneswar.He has numerous successful treatment cases registered in his name. 

Downloads

Published

2025-04-01

Issue

Vol. 21 No. 1 (2025): Al-Kindy College Medical Journal

Section

Research Articles

License

Copyright (c) 2025 AL-Kindy College Medical Journal

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

How to Cite

1.
Uthansingh K, Mishra S, Pati BK. Assessment of VDR Gene Polymorphism (FokI) in Epithelial Ovarian Cancer Using PCR-RFLP Method. Al-Kindy Col. Med. J [Internet]. 2025 Apr. 1 [cited 2025 Apr. 2];21(1):26-31. Available from: https://jkmc.uobaghdad.edu.iq/index.php/MEDICAL/article/view/1842

Similar Articles

1-10 of 80

You may also start an advanced similarity search for this article.