Basal cell markers:34BE12 and p63, improving detection of basal cells in atypical prostatic lesions

Authors

  • Inas A. Rasheed

DOI:

https://doi.org/10.47723/kcmj.v13i2.95

Keywords:

BPH ,HG-PIN , prostatic carcinoma , basal cell markers, 34BE12 , HMW-CK , p63

Abstract

Background: The diagnosis of prostatic pathology may be of challenging , as some  difficult and suspected, atypical  cases may lack basal cell layer by routine H&E sections . Antibodies against 34BE12(HMW-CK) and p63 aid the diagnosis of such cases , to distinguish benign from  malignant prostatic lesions.

Objective: to identify basal cells in atypical prostatic lesions ,and distinguish benign from malignant prostatic lesions.

Type of the study:  A retro-spective  study.

Methods:  115cases of  paraffin embedded prostatic tissue blocks ,diagnosed as : 76 cases were benign prostatic hyperplasia( BPH) , 9 cases were  high grade –prostatic intraepithelial neoplasia (HG-PIN) , and 30 cases were prostatic carcinoma(PCa) .Sections from each blocks were prepared for immunostaining with 34BE12 and p63.

Results : basal cells were detected in cases of BPH , and HG-PIN , and absent  in all cases of prostatic carcinoma ,using basal cell markers . Negative benign glands(>2) were found in 71.6% and 38.2%  for   BPH  and 57.1% and 55.6% for HG-PIN immunostained  with high molecular weight cytokeratin (34BE12) and p63 , respectively, and significantly reduced to 9.0% and 11.1% for BPH and HG-PIN, respectively  with combined using of both markers .Conclusion : Combination of both   basal cell markers (34BE12 , p63) improving basal cell detection in atypical ,suspected prostatic lesions and distinguish benign from malignant lesions.

Author Biography

  • Inas A. Rasheed

    Received 7th Sept  2016,

    Accepted in final 22th May  2017

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Published

2019-04-18

How to Cite

1.
Rasheed IA. Basal cell markers:34BE12 and p63, improving detection of basal cells in atypical prostatic lesions. Al-Kindy Col. Med. J [Internet]. 2019 Apr. 18 [cited 2024 Nov. 21];13(2):56-8. Available from: https://jkmc.uobaghdad.edu.iq/index.php/MEDICAL/article/view/95

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