Basal cell markers:34BE12 and p63, improving detection of basal cells in atypical prostatic lesions

  • Inas A. Rasheed
Keywords: BPH ,HG-PIN , prostatic carcinoma , basal cell markers, 34BE12 , HMW-CK , p63

Abstract

Background: The diagnosis of prostatic pathology may be of challenging , as some  difficult and suspected, atypical  cases may lack basal cell layer by routine H&E sections . Antibodies against 34BE12(HMW-CK) and p63 aid the diagnosis of such cases , to distinguish benign from  malignant prostatic lesions.

Objective: to identify basal cells in atypical prostatic lesions ,and distinguish benign from malignant prostatic lesions.

Type of the study:  A retro-spective  study.

Methods:  115cases of  paraffin embedded prostatic tissue blocks ,diagnosed as : 76 cases were benign prostatic hyperplasia( BPH) , 9 cases were  high grade –prostatic intraepithelial neoplasia (HG-PIN) , and 30 cases were prostatic carcinoma(PCa) .Sections from each blocks were prepared for immunostaining with 34BE12 and p63.

Results : basal cells were detected in cases of BPH , and HG-PIN , and absent  in all cases of prostatic carcinoma ,using basal cell markers . Negative benign glands(>2) were found in 71.6% and 38.2%  for   BPH  and 57.1% and 55.6% for HG-PIN immunostained  with high molecular weight cytokeratin (34BE12) and p63 , respectively, and significantly reduced to 9.0% and 11.1% for BPH and HG-PIN, respectively  with combined using of both markers .Conclusion : Combination of both   basal cell markers (34BE12 , p63) improving basal cell detection in atypical ,suspected prostatic lesions and distinguish benign from malignant lesions.

Author Biography

Inas A. Rasheed

Received 7th Sept  2016,

Accepted in final 22th May  2017

Published
2019-04-18
How to Cite
Rasheed, I. (2019). Basal cell markers:34BE12 and p63, improving detection of basal cells in atypical prostatic lesions. AL-Kindy College Medical Journal, 13(2), 56-58. https://doi.org/10.47723/kcmj.v13i2.95