Association of HLA-DRB1/DQB1 Alleles and Genetic Susceptibility to Type 1 Diabetes Mellitus

Authors

  • Hiba Omer AbdelRhman Hussein Faculty of Graduate Studies and Scientific Research, National University, Sudan
  • Sababi Salih Abdalla Faculty of Medical Laboratory Sciences-National University, Sudan
  • Sakeena NourEldine Salih Pediatrics department-National University, Sudan, Pediatrics department -Alneelain University -Consultant Pediatrician in Bashair hospital, Sudan.
  • AbdElkarim Abdrabo Department of clinical chemistry-Faculty of Medical Laboratory Sciences-Al-Neelain University-Sudan
  • Mohamed Abdelgadir Mahdi Faculty of Medical Laboratory Sciences-National University, Sudan

DOI:

https://doi.org/10.47723/kcmj.v19i2.980

Keywords:

Type 1 Diabetes, HLA, DRB1/DQB1, Single nucleotide polymorphism

Abstract

Objective: This study was conducted to identify the association of HLA-DRB1/DQB1 genes with the susceptibility or resistance to type 1 diabetes mellitus (T1D) among patients between the ages of five and eighteen.

Subjects and Methods: The study included 200 Sudanese participants, ages ranging from 5 to 18. One hundred participants were healthy non-diabetic as the control group and 100 with T1D as the case group. The investigation was carried out in Khartoum state. The selection of patients with T1D was from diabetic centers and hospitals. The allele-specific-refractory mutation system-polymerase chain reaction (ARMS-PCR) technique was applied to identify the HLA gene polymorphism.

Results: There was a significant difference in genotype frequency across the groups in the current investigation (Kruskal-Wallis, p-value= 0.021). Whereas CG was not substantially different across groups (Chi-square, p-value=0.116), the CC genotype was considerably greater (46.0%) in patients (Chi-square Adjusted p-value0.001).

Conclusion: This study found that patients' genotypes and allele frequencies are significantly correlated when compared to those of healthy participants.

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Published

2023-08-30

How to Cite

Association of HLA-DRB1/DQB1 Alleles and Genetic Susceptibility to Type 1 Diabetes Mellitus. (2023). AL-Kindy College Medical Journal, 19(2), 202-206. https://doi.org/10.47723/kcmj.v19i2.980

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